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Thermo Scientific™ Power-Bind™ Streptavidin Coated Particles
Description
Bind a variety of biotinylated ligands, including DNA/RNA, oligos, antibodies and proteins with Thermo Scientific™ Power-Bind Streptavidin Coated Particles. Particles have very high and stable biotin binding capacities and address the concerns of diagnostic test kit manufacturers and nucleic acid researchers. The microparticles reduce serum matrix effects and improve assay accuracy, resulting in low, non-specific binding of serum proteins and other interfering substances.
The use of microparticles as a solid phase support in various immunoassays and affinity purifications has been known for some time; however, achieving high activity and stable binding of solid phase ligands has been a major difficulty. Biotinylated compounds become stably bound to Power-Bind Streptavidin Coated particles after simple incubation in buffer due to the high affinity of the biotin-streptavidin interaction. The particles also function as a spacer, which improves the specific activity of the bound ligand. Monodisperse nonmagnetic particle suspensions with streptavidin covalently bound to the surface in a highly active form
Features:
· Made from 0.3µm or 0.8µm Thermo Scientific™ OptiLink™ Carboxylate-Modified particles
· High biotin-binding capacity
· Extremely stable, long shelf life
· Simplifies the process of binding ligands to particles
· Combines the advantages of high surface area and easy, high affinity, high specific activity binding
· Excellent for chemiluminescent assays, biotinylated ligand capture, and for molecular biology, immunoassay, or sample prep applications
Benefits:
· Dissociation constant (Kd 10-15 molar), easy one-step binding protocols for biotinylated ligands
· High activity of surface bound ligands to easily solve difficult coupling problems
· Simple aqueous biotinylation reactions
· Low nonspecific interactions (blocked microparticle surface)
· Highly mobile particles for membrane-based applications
· Can be used in EIA formats with biotin/enzyme detection systems
· Beneficial spacer effect of SA molecule
· A choice of 0.3µm or 0.8µm diameters for different application requirements
Recommended for:
· Universal nonmagnetic bead for coupling unique ligands.
· Clinical diagnostics and molecular biology applications.
Applications
· Clinical diagnostic kit development
· Nucleic acid research
Specifications
|
Certifications/Compliance |
Includes Certificate of Analysis |
Includes |
Package Insert and Certificate of Analysis |
Sample Evaluation Packs |
Power-Bind evaluation samples are packaged in 1mL bottles at 1% solids (10 mg/mL). Select individual lots to evaluate. |
Stability |
Guanidinium thiocyanate; DMF and DMSO to 100% Sonication; environments PCR temperature cycling |
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|
|
Description |
Power-Bind Streptavidin Coated Particles |
Preservative |
0.05% Na Az |
Specialty Packaging |
Power-Bind Streptavidin Coated particles are available in different concentrations and package sizes upon request. |
Storage Requirements |
2° to 8°C |
|
Products
| Catalog Number |
Nominal
Diameter |
Bottle Size
|
%
Solids |
Binding
Capacity |
Mfr. No. |
| 09-981-075 |
0.3 μm |
1 mL |
1% |
~1000 pmol/mg |
2900-0701-011150 |
| 09-981-076 |
0.3 μm |
5 mL |
1% |
~1000 pmol/mg |
2900-0701-010150 |
| 09-981-077 |
0.3 μm |
100 mL |
1% |
~1000 pmol/mg |
2900-0701-010350 |
| 09-981-078 |
0.85 μm |
1 mL |
1% |
~1000 pmol/mg |
2900-1801-011150 |
| 09-981-079 |
0.85 μm |
5 mL |
1% |
~1000 pmol/mg |
2900-1801-010150 |
| 09-981-080 |
0.85 μm |
100 mL |
1% |
~1000 pmol/mg |
2900-1801-010350 |
