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Thermo Scientific™ Evolution™ 260 Bio UV-Visible Spectrophotometer
Description
Advance your research with reliable, absorbance results from the Thermo Scientific™ Evolution™ 260 Bio UV-Vis spectrophotometer, designed for the changing field of life science. With the choice of tablet or computer control, the Evolution 260 Bio spectrophotometer offers high-performance measurements for a variety of biological samples and measurements. Powerful software, precision optics, and an extensive line of accessories combine for a complete solution to move you from samples to answers faster. Thermo Scientific™ INSIGHT™ software, included with the Evolution 260 Bio system, offers pre-programmed assay methods for increased accuracy and convenience.
Complete Life Science Applications
Next generation INSIGHT software provides the streamlined convenience of easy-to-understand nucleic acid and protein analysis methods and includes dedicated modules for:
· Nucleic Acid concentration by direct UV measurement at 260nm including DNA, ssDNA, RNA, and siRNA
· Nucleic acid purity measurements from 260/280nm and 260/230 ratios
· Protein analysis by direct UV measurement at 205 or 280nm
· Most common colorimetric analysis methods including: Pierce 660nm Protein assay, Bradford, BCA and Lowry
· Measuring the fluorescence labeling efficiency for proteins and nucleic acids
· Measuring the microarray labeling efficiency with one or two dyes
Customizable, Straightforward INSIGHT Software
· Personalize your home screen for easy navigation and convenience
· Workbook tools save time and ensure accurate results
· CUE scripting allows creation of customized interface and simplified workflow
Best-in-Class Performance
· Double-beam geometry ideal for kinetics or any sample that may change over time
· Application Focused Beam Geometry (AFBG) technology provides superior results for microcells and turbid samples
· Xenon flash lamp requires no warm-up time allowing instant measurements
· Electronic triggering provides the highest level of accuracy for rapid-mixing kinetics measurements
· Precision monochromator drive delivers fast scanning data collection and wavelength accuracy Powerful Convenience at your Fingertips
· Color, touch screen provides instrument control using built-in computer
· Integrated keypad allows communication with INSIGHT software
· Choice of Local or PC configurations provides flexibility and convenience
Accessories for all your Sampling Needs
· Wide accessory selection with various temperature control options, sample changers, sippers and solid sampling devices complete your analysis
· Automated cell changers with Peltier temperature control increase sample throughput
· Choice of Peltier Single Cell Holder or a Smart 8-Cell Peltier system for temperature control and sample monitoring from 0° to 100°C
· Easy software integration gives you complete control of your measurements
Reliable Assurance of System Performance
· The Thermo Scientific UV Validator package provides support for system qualification and validation activities
· Complete documentation facilitates compliance of your system with the requirements of FDA, GxP, ISO 9001:2000 and ISPE 2001 guidelines and regulations
· CVC and Mercury Lamp accessory automated testing ensures accuracy of your data
· Thermo Scientific INSIGHTsecurity software provides complete tools to meet 21 CFR Part 11 requirements
Specifications
|
| Accuracy (Photometric) |
1A: ±0.006A 2A: ±0.010A Measured at 440nm using calibrated neutral density filters traceable to NIST |
| Beam Geometry |
Double-beam with Application Focused Beam Geometry |
| Data Interval |
10, 5, 2, 1.0, 0.5, 0.2, 0.1nm |
| Dimensions (L x W x H) |
62.2 x 48.6 x 27.9 cm (24 x 19 x 11 in) |
| Optical Design |
Double Beam with sample and reference cuvette positions; Application Focused Beam Geometry; Czerny-Turner Monochromator |
| Drift |
<0.0005 A/hr, 500 nm, 1.0nm SBW, 1 hr warm-up |
| Noise |
0A: <0.00015A
1A: <0.00025A
2A: <0.00080A
260nm, 1.0nm SBW, RMS |
| Photometric Accuracy Instrument |
1A: ±0.006A 2A: ±0.010A Measured at 440nm using calibrated neutral density filters traceable to NIST |
| Photometric Range |
>3.5A |
| Scan Ordinate Modes |
Absorbance, % Transmittance, % Reflectance, Kubelka-Munk, log (1/R), log (Abs), Abs*Factor, Intensity |
| Baseline Flatness |
±0.0010A, 200-800nm, 1.0nm SBW, smoothing |
| Certifications/Compliance |
ISO 9001:2008 |
| Detector Type |
Dual Silicon Photodiodes |
| Lamp |
Xenon Flash Lamp, 3-year warranty (7 years typical lifetime) |
| Spectral Bandwidth |
Variable: 1.0nm; 2.0nm; AFBG microcell optimized; AFBG fiber optic optimized; AFBG materials optimized |
| Electrical Requirements |
100-240 V 50-60 Hz selected automatically, 150W maximum |
| Pharmacopoeia Compliance Testing |
(Guaranteed Performance Specifications) Resolution (toluene in Hexane): ≥1.8A Photometric Accuracy (60mg/L K2Cr2O7): ±0.010A Stray Light: ≤1%T at 198nm: KCI; ≤0.05%AT at 220nm: Nal, KI Wavelength Accuracy: ±0.5nm 541.9, 546.1nm Hg emission lines, ±0.8nm full range Wavelength Repeatability: ≤0.05nm, repetitive scanning of 546.1nm Hg emission line |
| Photometric Display |
-0.3 - 4.0A |
| Photometric Repeatability |
±0.0002A |
| Scan Speed |
<1-6000nm/min, variable |
| Wavelength Accuracy |
±0.5nm (541.9, 546.1nm mercury lines) ±0.8nm (full range 190-1100nm) |
